FabULOUS™
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FabULOUS™ Fab Kit
Lyophilized enzyme and affinity resin for hinge-region digestion of murine IgG and purification of fragments
FabULOUS (SpeB) is a cysteine protease that digests in the hinge region of IgG from several different species and subclasses, generating Fab and Fc fragments.
FabULOUS Fab Kit contains one vial of FabULOUS enzyme with 2000 units for digestion of 2 mg murine IgG, and 4 CaptureSelect™ LC-Kappa (mur)* microspin columns for affinity purification of fragments.
The affinity matrix recognizes the LC-Kappa domain of murine IgG antibodies, which enables purification of Fab fragments. Each microspin column contains sufficient material to purify 0.5 mg IgG.
Why FabULOUS™ Fab Kit?
- Purification of Fab fragments from murine IgG
- Fast – pure fragments in under two hours
- Reliable workflow for preparative applications
Available Products
FabULOUS™ Fab Kit 2 mg
Lyophilized enzyme and affinity resin for above hinge digestion of 2 mg murine IgG and purification of fragments
For information on how to order, visit Place an Order or contact us directly at order@genovis.com.
Product Information
Unit Defintion
One unit digests ≥ 95% of 1 µg of human IgG1 in PBS pH 7.4 with 5 mM DTT or TCEP at 37˚C for 1 hour. This is also valid for 1 µg of mouse IgG1 in PBS with 30-50 mM L-cysteine as reducing agent.
Content and Storage
FabULOUS Fab Kit is shipped cold.The FabULOUS Lyophilized vials should be stored at -20°C upon arrival.
The microspin columns containing CaptureSelect™* LC-Kappa (mur) resin should be stored at +4-8°C. Do not freeze the spin columns!
After reconstitution, the FabULOUS enzyme is stable for 1 month at +4-8°C.
Resources
Instruction for Use
Product Specifications
Safety Data Sheet
Certificate of Analysis
Find your certificate of analysis »Performance Activity
Preparation of pure, homogeneous mouse IgG Fab fragments with no residual enzyme by combining FabULOUS digestion and affinity purification.
Citations
Powered by BiozFAQ and Support
Does FabULOUS need reducing condition to work?
Yes, FabULOUS requires reduced condition for activity and reaction is performed in the presence of 1-5 mM DTT or TCEP at neutral pH.
How can I stop the FabULOUS reaction?
FabULOUS enzyme reaction is inhibited with iodoacetamide, iodo acetic acid or lowering pH below 3.
Can I purify Fab fragments from human IgG with FabULOUS Fab kit?
No, FabULOUS Fab kit contains CaptureSelect LC-kappa (mur) spin columns with selective affinity for murine LC-kappa. If you need to generate pure Fab from human IgG1 you can use either FabALACTICA Fab Kit or GingisKHAN Fab Kit. If you have a different subclass of human IgG, please contact support@genovis.com for guidance.
Is it possible to prepare Fab fragments from different antibodies using GingisKHAN Fab kit?
Yes. GingisKHAN is provided lyophilized. After reconstitution, the enzyme can be used for up to 4 different antibodies. The digestion is performed in solution and you can prepare Fab fragments from 4 x 0.5 mg human IgG1. The purification step is done with Capture Select columns and 4 columns are provided in the kit.
I work with mouse IgG2a containing LC-lambda, can I use another affinity resin for purification of the Fab?
Yes. There is the possibility to exchange the CaptureSelect LC-kappa (mur) columns to columns containing CaptureSelect LC-lambda (mouse). This resin is specific for mouse IgG. Please contact support@genovis.com if this is of interest.
Legal & Disclaimers
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Genovis products are intended for research use only. They are not intended to be used for therapeutic or diagnostic purposes in humans or animals. All goods and services are sold subject to Genovis’ General Terms and Conditions of Sale.
* Thermo Scientific™ CaptureSelect™ resin from Thermo Fisher Scientific Inc. and its subsidiaries. Thermo Fisher and CaptureSelect are trademarks of Thermo Fisher Scientific Inc. and its subsidiaries.
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